Re: [DIYbio] help out a noob, marmite for malt medium?

9:54 AM |

Hi Micky,

welcome to the DIY community! =D

Marmite is not the conventional ingredient for your medium, but it should do the trick. You could additionally buy some malt extract, which is used by some home brewers.

Typical malt yeast agars contain:

Ingredients (g/L):
Peptic digest of animal tissue, 5.00
Yeast extract, 3.00
Malt extract, 3.00
Dextrose (Glucose), 10.00
Agar, 20.00

You might test this recipe and replace yeast extract by Marmite (probably a bit more than 3g/L) and some malt extract, maybe this one:
http://www.northernbrewer.com/shop/brewing/brewing-ingredients/malt-extract

You can buy glucose in a supermarket.

You might try some gelatin instead of agar.

Skip the peptic digest of animal tissue. I don't have a proper replacement for that in mind, but it should work without it.


I never tried a medium like that, but I'm interested whether it works, I don't know why it should not ^^


As for your glass ware: before using it, cover it with alufoil and put it into an oven for at least two hours at 160 °C.
You should also somehow sterilize your medium, propably simply by cooking? ._.

All other tools should be dipped in 70% Ethanol (if available) and burned before use. Also, you should put a bit of ethanol on your gloves and rub it dry before touching anything. ^^

Good Luck! I'm looking forward to your results! ;)

Cheers
Filip


2015-01-29 18:07 GMT+01:00 Micky Dembek <mwdembek@googlemail.com>:
Hello
Go easy on me please! 
I'm first year Natural Sciences student. I do it with Open University as this is the only way I can stay working and keep studying, it has it ups and downs. We do mostly NOT INTERESTING STUFF, but it's not only through computer as on science courses we actually get to go for lectures every now and then. The real down side is that i'm mostly interested in biology and genetics and we don't really do a lot of it on first year. I know that next year I will have lots of microbiology etc. Anyways i've seen an article on synthetic biology in a foreign newspaper and i've got interested straight away.  I have decided to set up my own lab and do my own experiments as I have vitualy no access to laboratory on OU and any kind 'scientific investigation' we do is not related to subjects i'm interested in. 

Since i knew nothing about laboratories i've done as much research as possible and I have ended up on this group, finding many interesting DIY websites and DIY guides (which stopped me from buying lots of expensive equipment without knowledge how to use them)

So i want to start my first culture and (obviously!) decided it's going to be a P.stripticus - yes just because it glows!

now i have found one research paper on P.stripticus and it states that for best growth and luminescence malt yeast agar medium should be used. Now (and please don't laugh. There are no stupid questions!:)   ) can marmite be used in this medium? As far as i'm aware it is a malt yeast extract....anyone  can hep me on this one?:)

I will set up a website to show results of any of my endavours with biology so noobs like me can have another great starting point :)





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