Re: [DIYbio] Re: Reviving bacteria from 1 year old petri dish

I'd let them recover for 30m/1h in SOC or LB, then plate on kanamycin,
yes. But, even after a year, if they were stable at 4C you should be OK.

I've seen 4C in an agar stab recommended as the best way to store
plasmid-bearing cells, preferable over -20C and possibly even -80C, in
at least one reputable source. But that's a stab, whereas in an
overgrown petri dish things might be less stable.

Try your luck, but if it's kanamycin do try a recovery period I think.

On 28/01/15 13:25, Mega [Andreas Stuermer] wrote:
> But then you have to breed them for an hour in LB without kanamycin first to give them time to produce the enzyme?
>

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