Re: [DIYbio] Re: Reviving bacteria from 1 year old petri dish

Well, we finally got tons of growth of the liquid medium directly plated onto a kanamycin agar plate. Without recovery.We put some colonies of this into LB kanamycin liquid medium and it grew cloudy.

Unfortunatly we don't have time till the workshop to re-do this from the 1 year old agar plate -> LB for recovery -> LB kan for selection.

Still, as it is kanamycin resistant I hope it is the correct bacterium

On Wed, Jan 28, 2015 at 3:00 PM, Cathal Garvey <cathalgarvey@cathalgarvey.me> wrote:

I'd let them recover for 30m/1h in SOC or LB, then plate on kanamycin, yes. But, even after a year, if they were stable at 4C you should be OK.

I've seen 4C in an agar stab recommended as the best way to store plasmid-bearing cells, preferable over -20C and possibly even -80C, in at least one reputable source. But that's a stab, whereas in an overgrown petri dish things might be less stable.

Try your luck, but if it's kanamycin do try a recovery period I think.

On 28/01/15 13:25, Mega [Andreas Stuermer] wrote:

But then you have to breed them for an hour in LB without kanamycin first to give them time to produce the enzyme?

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