Re: [DIYbio] Electronic requirements for redesign of Arduino PCR thermal cycler

Keeping the lid at a constant temp of ~90C will generally suffice. Most PCR machines hold the lid at 90C - 105C.

I have tried doing the lid at same temperature as the tubes but received condensation. I think this might have been because of the different heat transfer rates? Maybe you can figure it out?

Looking on eBay these days you can buy most of the parts for the Arduino thermalcycler for much cheaper than the $85USD pricetag (inexpensive Arduino boards &c). Also, keep on eye out on eBay for thermal cyclers. Sometimes people don't know what the actual equipment is so they just list it as brand and product names so searching directly for brand names help. I have found some for under $100 that work great though this is from the US, so who knows what you might find?



Josiah




On Thursday, February 26, 2015 at 3:02:09 PM UTC-8, Andy Morgan wrote:

Ah it all becomes clear, I was wondering why they listed a 12V power supply AND a regular power cable.

Thanks Simon.

On Friday, February 27, 2015 at 8:22:16 AM UTC+13, Simon Field wrote:

I presume the power supply is simply for the Arduino part of the project, which is unchanged from the original. In fact, the only thing that has changed is the heater. It's like swapping a 40 watt light bulb for a 100 watt light bulb. You don't have to change anything else.

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