RE: [DIYbio] Re: At home fabrication of micron scale microfluidics

This is true, only issue there was when using a syringe to pull from chip to chip, the line would sputter and induce air pockets on top of very swift changes in fluid flow once it gets past the bottleneck that is the chip. There is a lot of resistance since the channel features are so small that manual pull is to quick. Need a syringe pump in reverse that pulls slowly.

Like filling a syringe quickly with liquid through a very small needle, it bucks back until the liquid meets the top of the plunger (vacuum until pressure equalizes) so either way fine, non-manual control would be ideal. Backpressure is too high for standard cheap-o eBay peristaltic pumps in either direction. Basically its analogous to electronic theory...small channel, high resistance. Syringe acts as a voltage source trying to pull more than the channel can deliver so its either slow or the channels (or chip) fails structurally.

For the few moments when the pdms is perfectly bonded via coronal arch discharge and everything is aligned properly, I did get some plant protoplasts stuck in a T junction for a while while flowing media through. Its possible, just really really finicky.

On my laundry list of experiments to run, I'd like to do some data gathering on the characteristics of shrinky dink plastic sheets, temperature, shrink rate (~63%), etc to see if its a viable material for making multiple fairly-identical chips. Basically a datasheet characterizing the material within the scope of microfluidics. May prove useful to people trying to start working with microfluidics.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

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From: John Griessen
Sent: ‎2/‎27/‎2015 6:46 PM
To: diybio@googlegroups.com
Subject: Re: [DIYbio] Re: At home fabrication of micron scale microfluidics

On 02/27/2015 05:29 PM, Simon Quellen Field wrote:
> The alternative that suggests itself is to pull the liquid from one block to another using a relative vacuum. Now the liquid wants
> to go only where you want it to go.

because vacuum makes contact seals seal harder, and pressure makes them leak.

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