Re: [DIYbio] Re: help out a noob, marmite for malt medium?

On Sun, Feb 1, 2015 at 2:31 PM, Micky Dembeck
<genialnyzolw@googlemail.com> wrote:
> yes i know petris don't look great with all the bubbles but i have made

Wow, lots of bubbles! I wonder if you didn't cool the media long
enough after sterilizing it... did you let the pressure cooker return
from 15 PSI to atmospheric without removing the pressure weight? When
you are ready to pour the plates, the media should be cool enough that
if you spilled it on your skin you wouldn't be burned (or conversely,
that the jar containing the media is cool enough to hold with a
bare/thinly-gloved hand).

The bubbles make it extremely hard to tell if you've got sub-surface
growth happening. Were the petris also sterile?

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