Re: [DIYbio] Re: help out a noob, marmite for malt medium?

On Mon, Feb 2, 2015 at 1:49 PM, Micky Dembeck
<genialnyzolw@googlemail.com> wrote:
> W dniu poniedziałek, 2 lutego 2015 19:01:26 UTC użytkownik Nathan McCorkle
>> Wow, lots of bubbles! I wonder if you didn't cool the media long
>> enough
>
> Yes it was cool enough, the reason for bubbles was simply that agar start to
> solidify before i even had the chance to pout it. so in panic I have shaked
> the jar well and pour it straight in. :)

Ahh, so I guess 'too cool' maybe. If this happens in the future, a few
10-second bursts in a microwave should fix things up! (but definitely
only do bursts in the microwave, if the agar boils it bubbles a LOT
and can erupt out of the container, and when you realize and open the
microwave, the eruption can suck back into the container, taking
contaminants with it)

>> Were the petris also sterile?
>
> Yes I have baked everything for a long time in 150 C oven, wrapped in
> aluminium foil to prevent contamination when i took them out from the
> oven...i tried to do everything as per guides I can find in internet :)

Cool, sounds good. Did you not use a pressure-cooker then? Moisture
conducts a lot more heat, so that could be something that might cut
off some sterilization time. For things like petri dishes, you can
either bake or pressure cook them dry, but the last few times I
cleaned glass for culture, I added just a bit of water... this way I
know there is sufficient heat conduction inside them, and also I have
extra sterile water on hand in case I need it.


> today "IT" got a bit bigger and i can already clearly see it grows
> upwards...(i say 'IT" because i'm still not sure if I actually suceeded.)

Congrats! Next you might try cloning a mushroom you buy at the grocery
store, oyster mushrooms have fruited for me on PDA alone, with barely
any care provided to them (but I also never opened those to prevent
any sort of contamination).

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