W dniu czwartek, 29 stycznia 2015 17:07:31 UTC użytkownik Micky Dembek napisał:
HelloGo easy on me please!I'm first year Natural Sciences student. I do it with Open University as this is the only way I can stay working and keep studying, it has it ups and downs. We do mostly NOT INTERESTING STUFF, but it's not only through computer as on science courses we actually get to go for lectures every now and then. The real down side is that i'm mostly interested in biology and genetics and we don't really do a lot of it on first year. I know that next year I will have lots of microbiology etc. Anyways i've seen an article on synthetic biology in a foreign newspaper and i've got interested straight away. I have decided to set up my own lab and do my own experiments as I have vitualy no access to laboratory on OU and any kind 'scientific investigation' we do is not related to subjects i'm interested in.Since i knew nothing about laboratories i've done as much research as possible and I have ended up on this group, finding many interesting DIY websites and DIY guides (which stopped me from buying lots of expensive equipment without knowledge how to use them)So i want to start my first culture and (obviously!) decided it's going to be a P.stripticus - yes just because it glows!now i have found one research paper on P.stripticus and it states that for best growth and luminescence malt yeast agar medium should be used. Now (and please don't laugh. There are no stupid questions!:) ) can marmite be used in this medium? As far as i'm aware it is a malt yeast extract....anyone can hep me on this one?:)I will set up a website to show results of any of my endavours with biology so noobs like me can have another great starting point :)Hello
It's been a while and I was busy at work + GFs family came over for 2 weeks so I did not even look inside my incubator.
good news is: something has grown on each plate!
even better news: two plates looks like are occupied by P.stripticus
Two plates I have set up with just yeast very successful
The original two plates have grown something that looks more like lichen, they stopped their growth probably due to lack of water.
One plate is covered by something black with white 'web' growing underneath perhaps someone can identify this?
Noob question 1 : if growth have stopped can this be restarted by adding a little bit of water?
Noob question 2 :What is the best way to identify what has grown on the plates? I have access to microscope so more picture to come.
Noob question 3: can P.stripticus be grown bigger from only what I have on plates? providing I will supply all the necesary substances, I have noticed that i can be grown on piece of wood. I want to create glowing shroom box for my brother as decoration for his new sushi place :)
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