So I've downloaded vector 1302 for snap gene and have my gene of interest but missing the understanding of how to cut the plasmid with either CRISPR or GB 2.0 for my minipreps... I guess my questions are how to i choose my sites for cleavage and how do I choose the promoter/terminator for GB for insertion into my binary vector?
I'd love a simple step by step. my current understanding is such:
- GOI -> E. coli
-- I'd love a simple step by step. my current understanding is such:
- GOI -> E. coli
- denature E. coli -> PCR
- ?
- Mini prep -> Agro via freeze/thaw
- Mini prep -> Agro via freeze/thaw
- plant transformation
GB has me reading on IIS restriction enzymes for binary assembly, though now that I'm playing with snapgene and have my destination vector I don't know what to do. HALP!!
Thanks,
GB has me reading on IIS restriction enzymes for binary assembly, though now that I'm playing with snapgene and have my destination vector I don't know what to do. HALP!!
Thanks,
Nico
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