Hi,
Your plant cell only understands monocistrons (bacteria express operons - multiple proteins from one promoter). So you either add IRES sites inbetween the proteins, or you add one promoter per gene.
Best,
Andreas
On Monday, April 27, 2015 at 3:13:19 PM UTC+2, Bijan wrote:
-- Your plant cell only understands monocistrons (bacteria express operons - multiple proteins from one promoter). So you either add IRES sites inbetween the proteins, or you add one promoter per gene.
Best,
Andreas
On Monday, April 27, 2015 at 3:13:19 PM UTC+2, Bijan wrote:
First off, I've only taken freshman biology, I have no idea what I am doing at a professional level.
Alright, I think I have an idea for making glowing plants. I was thinking I could use this -> http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens# With a 35S promoter (Which I can illegally get with some GMO food if I can find the right primers). Ill take the promotor and the Lux cassete from somewhere and insert it into the agro t-area which will inject it into the plant DNA.Beneficial_uses
What I don't know is
1.) How to get the 35S promoter primers
2.) How to stick the promotor on the luxABCD gene.
3.) How to isolate the t-area and integrate it into the phage cell.
I just put this together myself. But it seems possible right?
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