Hello!
I want to make more potent rodenticide from Salmonella enterica ssp. enteriditis var. Issatchenko (non-pathogenic for humans and other mammals - rodents only. It is used in rodenticide called "Зообакцид", from which it was isolated) by "learning" it to use lactose as carbon source (Salmonella sp. can not use lactose). I presuppose, what E. coli is quite similar with Salmonella sp. in genetic plan and these genes will work in it. So, I need lactose operon from E. coli or at least LacZ and LacY genes from it. I have BSL 2 lab at Moldova State University and rats from vivarium for this work, so it will be secure.
Can anybody send me a plasmid with needed genes?
Thanks for help!
-- I want to make more potent rodenticide from Salmonella enterica ssp. enteriditis var. Issatchenko (non-pathogenic for humans and other mammals - rodents only. It is used in rodenticide called "Зообакцид", from which it was isolated) by "learning" it to use lactose as carbon source (Salmonella sp. can not use lactose). I presuppose, what E. coli is quite similar with Salmonella sp. in genetic plan and these genes will work in it. So, I need lactose operon from E. coli or at least LacZ and LacY genes from it. I have BSL 2 lab at Moldova State University and rats from vivarium for this work, so it will be secure.
Can anybody send me a plasmid with needed genes?
Thanks for help!
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