Re: [DIYbio] Obtaining J. lividum or another non-pathogenic violacein producer

Hi Alan,

Officially to get the biobrick you have to be registered as a iGEM lab. To register the iGEM lab you need to affiliate to an institution/community/company.

Usually iGEM lab are really helpful. Find the closest iGEM lab and ask them to get the biobricks for you.

May I ask what is it your purpose?

Cheers,
Claudio

On Thursday, May 7, 2015 at 8:44:50 PM UTC+2, Alan WiIliams wrote:
Hi,
So I might play with the pathway later, but for right now I would like a working plasmid.  The biobrick looks fine for my purposes, does anyone know how to get those as an individual?
Thanks,
Alan



On May 7, 2015, at 1:53 PM, Brian Degger <brian....@gmail.com> wrote:

Nathan, 
Yes, you are right. It was bits for a pathway, not a plasmid as such.
However, I wasn't sure if Alan wanted to play with the pathway. I have also have also seen that http://parts.igem.org/Part:BBa_K274002?title=Part:BBa_K274002 produces violacein from l-tryptophane in E.coli  

On Thu, May 7, 2015 at 6:17 PM, Nathan McCorkle <nmz...@gmail.com> wrote:
On Thu, May 7, 2015 at 6:04 AM, Brian Degger <brian....@gmail.com> wrote:
> Last year, synbiota/genomicon was shipping the violacein pathway
> (https://synbiota.com/dna-market/8) for transforming into e. coli k12

Wasn't that more of a kit to try hacking the pathway into working,
rather than a plasmid with a working pathway?

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