Why is fluorescence difficult to measure? qPCR machines use lasers or LEDs to excite fluoropores and sensors to detect the fluorescence. I don't think phosphorescence is suitable for real time PCR. The advantage of fluoresence is its speed in excitation and emission. With each cycle, you can detect the amount of the target gene.
Since phosphorescence emission lasts longer, the emission from the previous excitation will interfere with later detection. Hence, it's unsuitable for continuous detection.
On Wed, May 6, 2015 at 3:14 AM, Gabriele Borelli <borelli.g92@gmail.com> wrote:
Hello,
I'm studying real-time PCR and I have a question regarding the dyes. I found on the Internet different types of this molecules, but all of them use the fluorescence. In my opinion the fluorescence is very fast and, from an electronic point of view, is difficult to measure. If a dye based on phosphorescence existed, it would be simpler. Do you know a phosphorescencent dye?
Best regards,
Gabriele
--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/a2269a32-d144-42dd-8da2-c5a0fd5813c6%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
--
In necessariis unitas, in dubiis libertas, in omnibus caritas.
-Marco Antonio Dominis
-Marco Antonio Dominis
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAAhF0RJGtxkgaxe%2BZBXOFukht0YyHQxgPYg8pDToKBAgE1NVpw%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment