Re: [DIYbio] Re: Anybody has E.coli lactose operon in a plasmid?

On Tue, May 26, 2015 at 11:09 AM, Dorif <dorif11@gmail.com> wrote:
> Well, virtually I don't need entire operon for this. It is enought to insert
> LacZ and LacY. If LacZ is functional with deletion in it - it is reaaly good
> idea to use this "cutted down" LacZ to minimize the insert size.

I think Matt's point was that in common plasmids, the lacZ is not
complete... this is for alpha-complementation, since the lacZ gene
product (the protein) is a tetramer composed of two dimers, one of
which is alpha and the other omega... so these common plasmids usually
lack one half of the lacZ gene (which I'm guessing is actually
polycistronic, e.g. two genes in one).

See here for a nice paragraph and graphic of the protein subunits:
http://www.ncbi.nlm.nih.gov/pubmed/23011886

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