Re: [DIYbio] Re: Biohacking gray hair

Thank you. Great paper! Here is the NYTimes take on it. 

http://www.nytimes.com/2015/04/24/health/chinese-scientists-edit-genes-of-human-embryos-raising-concerns.html?hp&action=click&pgtype=Homepage&module=second-column-region&region=top-news&WT.nav=top-news&_r=0

On Thu, Apr 23, 2015 at 8:42 AM, Gavin Scott <gavin@learn.bio> wrote:

On Thu, Apr 23, 2015 at 9:52 AM, Gordana Ostojic
<gordana.n.ostojic@gmail.com> wrote:
> Did anyone see that Chinese scientists used CRISPR to modify human embryo?

Seems like there's a long way to go before anyone will try this for real:

"We found that CRISPR/Cas9 could effectively cleave the endogenous
β-globin gene (HBB). However, the efficiency of homologous
recombination directed repair (HDR) of HBB was low and the edited
embryos were mosaic. Off-target cleavage was also apparent in these
3PN zygotes as revealed by the T7E1 assay and whole-exome sequencing.
Furthermore, the endogenous delta-globin gene (HBD), which is
homologous to HBB, competed with exogenous donor oligos to act as the
repair template, leading to untoward mutations. Our data also
indicated that repair of the HBBlocus in these embryos occurred
preferentially through the non-crossover HDR pathway. Taken together,
our work highlights the pressing need to further improve the fidelity
and specificity of the CRISPR/Cas9 platform, a prerequisite for any
clinical applications of CRSIPR/Cas9-mediated editing."

Their paper is open access at:
http://link.springer.com/article/10.1007%2Fs13238-015-0153-5

G.

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