Re: [DIYbio] Re: Promoter Testing

On Thu, May 14, 2015 at 5:42 PM, Linden <ff.lindenberg@gmail.com> wrote:
> Sure, I dont have it with me right know, but it was something like this:

So does that mean you only tested a single promoter? It would really
make more sense and be easier if you grew up multiple strains in the
same manner, with different promoters, then compared them to each
other. Otherwise, you'll need to look up published data and compare
using their methods.

You could also purify the GFP with, either ion-exchange chromatography
columns (pretty cheap and easy), or HPLC, etc... then you know the
elution volume, but not the concentration... but since it should be
pretty 'pure' you can compare to published fluorescence values to get
the concentration. When you know the concentration and the starting
volume of GFP cell culture, along with the cell-density of the
solution, you can get a value for GFP per cell.

If you really just want to compare promoters, either do what I said
and test them side-by-side... otherwise you need to replicate
previously published methods otherwise comparing to them won't make
sense.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9LS%3DVu%2BCUMerzsG6g%2BNpKG23asMd3c6AdKVao4zQ5uVrg%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.