Yes, it remains in exactly the same position relative to the operator. I think it is preferable to use Salmonella phage promotor for expression in Salmonella, doesn't it?
2015-05-30 14:01 GMT+03:00 Mega [Andreas Stuermer] <masterstorm123@gmail.com>:
Extremely hard to say... I just know from T7 if you space lacO (or was it tetO) more than 5 bp away it doesn't work anymore because the DNA helix. The repressor will bind the operator but in a different angle... Have you made sure that the core promoter will be in the same position relative to the operator? Isn't it possible to take the native coli one?--
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