Hi guys,
-- At the OpenWetLab in Amsterdam we did something similar to what Cathal suggested above.
Using calcium carbonate helps indeed isolating acetic acid producing strain, but as pH of the medium goes up, you will see pretty much the zygomyces taking over. You can spot them by their white colonies that create sort of a "bump" on the agar plate.
G.xylinus colonies are brownish also in my case. But I could spot them (after 3-4 days at 30°C) just if I added EtOH to the medium.
My suggestion is to add 4-6% alcohol to the medium, this will slow down yeasts and speed up Gluconacetobacter metabolism.
Be careful to streak the isolated colonies at least 3-4 times. Cleaning the strain from other microorganisms isn't always straightforward.
Best,
Fede
Il giorno domenica 7 giugno 2015 19:13:47 UTC+2, Philipp Kollenz ha scritto:
Il giorno domenica 7 giugno 2015 19:13:47 UTC+2, Philipp Kollenz ha scritto:
Hi thereI am trying to produce nanocellulose and the cultures you can buy on websites like this one (https://www.dsmz.de/catalogues/details/culture/ ) are too expensive. I heard it is part of a microbe mix that is used to make some kind of asiatic tea beverage called Kombucha. Is it possible to isolate it from there?DSM-2325.html best regards,Phil
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