What's the size difference between the target fragment and the unwanted larger fragment? You could run one PCR and isolate your target fragment. Then use that DNA as template for a second PCR amplification.
On Sun, Jun 14, 2015 at 10:20 PM, Linden <ff.lindenberg@gmail.com> wrote:
Hey guys,--I am designing a 40 bp primer (it is for homologous recombination in yeast), but part of it also anneal at another part of the plasmid, just like the drawing attached. Could it be a big issue? I dont have another plasmid with this gene..Thanks a lot!
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