Re: [DIYbio] Primer

What's the size difference between the target fragment and the unwanted larger fragment? You could run one PCR and isolate your target fragment. Then use that DNA as template for a second PCR amplification.

On Sun, Jun 14, 2015 at 10:20 PM, Linden <ff.lin...@gmail.com> wrote:

Hey guys,

I am designing a 40 bp primer (it is for homologous recombination in yeast), but part of it also anneal at another part of the plasmid, just like the drawing attached. Could it be a big issue? I dont have another plasmid with this gene..

Thanks a lot!

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