Re: [DIYbio] Primer

On Mon, Jun 15, 2015 at 2:00 PM, Linden <ff.lindenberg@gmail.com> wrote:
>
> Thanks, Phillyj
>
> Maybe I could digest it out of the plasmid, run an agarose gel, purify and then amplify. Probably ok, right?
>

I don't know if you want to digest the plasmid and then PCR. It might
be easier to do PCR on the plasmid and then cut out the smaller
(Target) band. I am assuming that you get 2 bands with good
resolution. You may end up with a smear on the gel (after 1st PCR) so
then maybe a plasmid digest would help isolate the specific region you
want.

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