Hey friends after serious work we finally annotated Plasmid vector with synthetic coagulation factor VIII gene in it,
On Saturday, January 17, 2015 at 7:31:14 AM UTC+5:30, Sunil Phani wrote:
-- anyone has the facility to synthesize it for me, please
I will share the annotation in any format per requirement
Thanks a Ton
On Saturday, January 17, 2015 at 7:31:14 AM UTC+5:30, Sunil Phani wrote:
can someone please help me setup DIY-BIO home Lab to perform mammalian cell culture to produce Recombinant Factor VIII protein with mammalian cells(CHO, HKE 293, HEL, etc) and kindly provide me recombinant dna protocols for identification, isolation , extraction, insertion and expansion .my requirement is to prepare pilot project in small scale DIY-BIO lab which can further be scaled.
if possible provide me vectors, cell line and seedstock. i am new to DIY-BIO hence i need helping hands of veterans like you ppl .. your help be highly appreciated
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