Huh, interesting thought. As long as they aren't also medicated for secondary bacterial infection (as some are)?
The powders are mostly talc, so unlikely to affect the agar formula much. Would need to be added after sterilising though, and are topical (ie designed for unbroken skin) formulas guaranteed sterile if unopened?
On 2 July 2015 08:35:11 GMT+01:00, Patrik D'haeseleer <patrikd@gmail.com> wrote:
Any recommendations for a good antifungal that could be used in such situations? Something that's going to inhibit all the yeast and molds from growing, but won't affect the bacteria.I keep wondering if some of the usual athlete's foot / jock itch products can be used for this, as an easily accessible DIY solution...Patrik
On Wednesday, July 1, 2015 at 1:31:57 PM UTC-7, Cathal Garvey wrote:IIRC (it was a while ago) G.xylinus is a dark brown and has little
distinctive shape; flat and brownish sounds about right. The
glossy/clear partner sounds like it might be a yeast.
Take pickings from distinct, separate examples of both colonies and
streak them out again on separate agar, you'd expect the brown guys to
*prodigiously* clear the CaCO3 in the agar and make it
clear/see-through, and in late-stage culture (+7 days or even later) to
see chunks of cellulose forming near, though not necessarily *on*,
colonies. That was my experience, at least.
As for the white guys, check 'em under a microscope; if they're yeast,
they're probably much larger than bacteria and easy to observe, for
starters. You might even see nuclei without a stain, though try
methylene blue for a cheap, cheerful DNA stain.
On 01/07/15 20:47, Philipp Kollenz wrote:
> I have two different types of colonies:
> - Flat and brownish
> - "Bumpy", glossy, and almost clear
>
> They both aren't yeasts (as far as I could tell with my microscope) and
> grew after 5-6 days at 26°C
> Which of them is the one I'm looking for?
>
> Am Sonntag, 7. Juni 2015 19:33:09 UTC+2 schrieb Cathal (Phone):
>
> It's easy to isolate from Kombucha, and some literature suggests
> this strain gives the best cellulose, too. :)
>
> Just make up "Gluconobacter agar" (look it up on thelabrat.com
> <http://thelabrat.com>) and streak your kombucha culture over it
> thinly. When I isolated it in this way, my colonies were brown,
> cleared the CaCO3 quickly, and in *late* culture, white crystals (of
> cellulose?) appeared around, but rarely atop, colonies.
>
> On 7 June 2015 12:37:54 GMT+01:00, Philipp Kollenz
> <philipp...@googlemail.com <javascript:>> wrote:
>
> Hi there
>
> I am trying to produce nanocellulose and the cultures you can
> buy on websites like this one
> (https://www.dsmz.de/catalogues/details/culture/ DSM-2325.html
> <https://www.dsmz.de/catalogues/details/culture/ DSM-2325.html>)
> are too expensive. I heard it is part of a microbe mix that is
> used to make some kind of asiatic tea beverage called Kombucha.
> Is it possible to isolate it from there?
>
> best regards,
> Phil
>
>
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