Re: [DIYbio] Re: help me setup DIY-BIO home Lab to produce Recombinant Factor VIII

Hi Sunil, have you thought of using arcturus.io (Arcturus Biocloud)? We're currently planning on making open source human insulin in E.Coli and the team there could help you with that (as well as setting up a team page to get advice).

I highly recommend checking them out and it'll be a lot cheaper and faster than setting up your own lab (they can mail you your construct and you can watch it online as the robot makes it).

Best,
Ryan

On Friday, July 3, 2015 at 9:42:42 AM UTC-7, Sunil Phani wrote:

Hey Mega after serious work we finally annotated Plasmid vector with synthetic coagulation factor VIII gene in it, 

anyone has the facility to synthesize it for me, please

I will share the annotation in any format per requirement  

Thanks a Ton

Sunil Phani

On Mon, Jan 26, 2015 at 3:43 PM, Mega [Andreas Stuermer] <masters...@gmail.com> wrote:

While the pVIB plasmid is shipping, you might want to look into immunology a bit (though it is a very big and complex field), what would happen if you injected crudely purified protein into a human. What are the negative effects, and how are they induced.

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