Re: [DIYbio] Re: help me setup DIY-BIO home Lab to produce Recombinant Factor VIII

Very topical:
http://www.economist.com/blogs/freeexchange/2015/07/greeces-economy-under-capital-controls

The EU is crushing Greece under boot-heel, and among the earliest
victims are Thyroxine and Insulin-dependent Greeks.

I wrote about the need for DIY Insulin/Thyroxine years ago and it's
still relevant; people underestimate how brittle the supply chains of
advanced medicine really are:
http://www.indiebiotech.com/?p=135

Given the difficulty of purifying blood-safe Insulin, Thyroxine (which
is orally bioavailable) might be a more immediately valuable project.
But with Sothic at IndieBio EU aiming to make Limulus Amoebocyte Lysate
(LAL) far, far more plentiful in the near future, perhaps LAL-testing of
stuff for safe injection will become more commonplace? I'm imagining a
market route something like:

1. DIY LAL test becomes available for intravenous drug users as part of
a harm-reduction strategy (GOOD IDEA)
2. Test gets re-appropriated to test batches of purified Factor VIII or
Insulin for human use, off-grid, by people who cannot access safe
medicine otherwise (Greece, much of India, for example).

Interesting times ahead.

On 05/07/15 01:36, Biotech Ryan wrote:
> Hi Sunil, have you thought of using arcturus.io (Arcturus Biocloud)?
> We're currently planning on making open source human insulin in E.Coli
> and the team there could help you with that (as well as setting up a
> team page to get advice).
>
> I highly recommend checking them out and it'll be a lot cheaper and
> faster than setting up your own lab (they can mail you your construct
> and you can watch it online as the robot makes it).
>
> Best,
> Ryan
>
> On Friday, July 3, 2015 at 9:42:42 AM UTC-7, Sunil Phani wrote:
>
>     Hey Mega after serious work we finally annotated Plasmid vectorwith
>     synthetic coagulation factor VIII gene in it,
>     anyone has the facility to synthesize it for me, please
>     I will share the annotation in any format per requirement
>
>     Thanks a Ton
>     Sunil Phani
>
>     On Mon, Jan 26, 2015 at 3:43 PM, Mega [Andreas Stuermer]
>     <masters...@gmail.com <javascript:>> wrote:
>
>         While the pVIB plasmid is shipping, you might want to look into
>         immunology a bit (though it is a very big and complex field),
>         what would happen if you injected crudely purified protein into
>         a human. What are the negative effects, and how are they induced.
>
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