Re: [DIYbio] Re: help me setup DIY-BIO home Lab to produce Recombinant Factor VIII

well said Cathal Garvey grate to be hearing your straight forward opinion from you,

i am really a grate admirer of your work as a matter of fact yesterday only i referred 

your article on this same topic in arcturus.io OSInsulin forum 

yes you are correct one has to prioritize research activities on vital issues first like

Factor VIII and Insulin/Thyroxine which are among the WHO recognized life saving drugs 

yet most of these drugs are propitiatory labeled only neither there is any generic 

formulation nor any major opensource project under progress. and these opensource 

projects too need to take up some bold innovative steps rather than thinking 

conservatively always like old school pharma companies we don't have any 

API's to protect 

hoping for democratization in its true essence 

On Monday, July 6, 2015 at 12:18:46 AM UTC+5:30, Cathal Garvey wrote:

Very topical:
http://www.economist.com/blogs/freeexchange/2015/07/greeces-economy-under-capital-controls

The EU is crushing Greece under boot-heel, and among the earliest
victims are Thyroxine and Insulin-dependent Greeks.

I wrote about the need for DIY Insulin/Thyroxine years ago and it's
still relevant; people underestimate how brittle the supply chains of
advanced medicine really are:
http://www.indiebiotech.com/?p=135

Given the difficulty of purifying blood-safe Insulin, Thyroxine (which
is orally bioavailable) might be a more immediately valuable project.
But with Sothic at IndieBio EU aiming to make Limulus Amoebocyte Lysate
(LAL) far, far more plentiful in the near future, perhaps LAL-testing of
stuff for safe injection will become more commonplace? I'm imagining a
market route something like:

1. DIY LAL test becomes available for intravenous drug users as part of
a harm-reduction strategy (GOOD IDEA)
2. Test gets re-appropriated to test batches of purified Factor VIII or
Insulin for human use, off-grid, by people who cannot access safe
medicine otherwise (Greece, much of India, for example).

Interesting times ahead.

On 05/07/15 01:36, Biotech Ryan wrote:
> Hi Sunil, have you thought of using arcturus.io (Arcturus Biocloud)?
> We're currently planning on making open source human insulin in E.Coli
> and the team there could help you with that (as well as setting up a
> team page to get advice).
>
> I highly recommend checking them out and it'll be a lot cheaper and
> faster than setting up your own lab (they can mail you your construct
> and you can watch it online as the robot makes it).
>
> Best,
> Ryan
>
> On Friday, July 3, 2015 at 9:42:42 AM UTC-7, Sunil Phani wrote:
>
>     Hey Mega after serious work we finally annotated Plasmid vectorwith
>     synthetic coagulation factor VIII gene in it,
>     anyone has the facility to synthesize it for me, please
>     I will share the annotation in any format per requirement
>
>     Thanks a Ton
>     Sunil Phani
>
>     On Mon, Jan 26, 2015 at 3:43 PM, Mega [Andreas Stuermer]
>     <masters...@gmail.com <javascript:>> wrote:
>
>         While the pVIB plasmid is shipping, you might want to look into
>         immunology a bit (though it is a very big and complex field),
>         what would happen if you injected crudely purified protein into
>         a human. What are the negative effects, and how are they induced.
>
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--
Scientific Director, IndieBio EU Programme
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