I don't think you will get very good results by starting a second day culture at the OD that you left off at. Like Sebastian mentioned, the condition of the cells matter. Say you stopped on day 1 during log phase at an OD of 0.8, and then on day 2 diluted a stationary phase culture to OD of 0.8. These cultures are not going to be the same. Probably the biggest factor is that a stationary phase culture will take some time (lag phase) to switch into growth mode. But also the nutrient content may be different (i.e. a culture that has been growing in and using the media for 8 hours vs one that was just put in the fresh media).
On Saturday, August 15, 2015 at 9:37:02 PM UTC-5, Linden wrote:
-- On Saturday, August 15, 2015 at 9:37:02 PM UTC-5, Linden wrote:
Hey guys!I would like to know if an E. coli, growth curve can be created in two steps. So on the first day I would start at OD 0.1 and measure it for 8 hours, and on the second day, I would start the culture at the OD it stopped the day before and measure for another 8 hours. Is it ok to do it?I really appreciate your insight!
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