Re: [DIYbio] Pilot Plant - Curiosity

On Tue, Aug 11, 2015 at 6:49 PM, Linden <ff.lindenberg@gmail.com> wrote:
> Hey guys,
>
> Does anyone know how inducers are used in a pilot plant, or if they are used
> at all? Let's say I have an E. coli with an inducible promoter, for example,
> ParaBAD or Plac, In a pilot plant it would be required a large amount of
> Arabinose or IPTG. Is it economically viable? Does it work?

Seems like IPTG might only be useful for protoplasts... not sure on
the specifics, but it was mentioned in a paper I just browsed through:

A steroid-inducible gene expression system for plant cells
MARK SCHENA, ALAN M. LLOYD, AND RONALD W. DAVIS
August 8, 1991
http://www.pnas.org/content/88/23/10421.full.pdf


In another paper, which I couldn't get, it says:
"
Here, protocols will be presented to work with five different
inducible systems: AlcR/AlcA (ethanol inducible); GR fusions, GVG, and
pOp/LhGR (dexamethasone inducible); XVE/OlexA (β-estradiol inducible);
and heat shock induction.
"

Inducible Gene Expression Systems for Plants
Lorenzo Borghi
Date: 28 Jun 2010
http://rd.springer.com/protocol/10.1007%2F978-1-60761-765-5_5


Here's a paper which looks good too, and I could get the PDF:
Promoters that respond to chemical inducers
Christiane Gatz and Ingo Lenk
1998

http://ac.els-cdn.com/S1360138598012874/1-s2.0-S1360138598012874-main.pdf?_tid=ed2dfde2-4118-11e5-b0e3-00000aab0f02&acdnat=1439401281_c1f7f25c259b4ef1afbf135734cb119f

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