Re: [DIYbio] Re: I want to test my own food - where do I start

> I'm rather curious as to how one would go about testing for whether or not
> random genes were manually tweaked (and not naturally mutated/introduced
> through bacterial/viral horizontal gene transfer) in the first place.

Any transgenic sequences can be detected by PCR or, even better
RT-PCR. You would purify DNA from the plant, design primers for the
specific sequences you are looking for, do the PCR and then run it out
on an agarose gel.

Most approved traits will have the CaMV 35S promoter sequence so you
can use primers for that promoter. Although, it's not guaranteed to
be in every transgenic line. If you have a have a particular trait
you are looking for, use this website to find it. It will have links
to detection information that includes details of the transgenic
sequences and suggested primer sequences.
http://www.isaaa.org/gmapprovaldatabase/

For example, if you're looking to detect Hercules RW maize
(Glufosinate tolerance and root worm resistance), the search function
will bring you to this page:
http://www.isaaa.org/gmapprovaldatabase/event/default.asp?EventID=112
which details this particular trait. Click on "Documents and Links",
then on "GMO Detection Method Database". That will take you to a page
with sequences, primers, recommended detection methods, etc. The
recommended detection methods tend to be primers and Taqman RT-PCR
probes.

-cory

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