Re: [DIYbio] short dsDNA

One method would be what Keoni suggested: buy two different ssDNA
oligos with complementary sequences, mix them together, heat to ~95C
for a bit and then cool. This will work well if your target length is
less than 60 bases. When you go past 60 bases the price per base goes
up significantly. You could also request they PAGE purify the oligos
which would give you a narrower distribution of lengths.

Another option is to use PCR to amplify a region of a template DNA
molecule that is exactly the length that you need. This will give you
a large amount of DNA for cheap. I'm not sure how accurate this is
relative to annealing ss oligos.

Or do what Simon suggested and cut a plasmid with a blunt-cutting
restriction enzyme. Assuming you have a plasmid with restriction
sites correctly spaced, this will give you the most DNA for your
dollar. But you'll need to gel purify which is laborious if you're
handling a large amount of DNA.

It really depends on how much DNA you need and how much precision is
required. What percent of molecules must be the right length? 99?
99.9?

-cory

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