[DIYbio] Re: [New post] Ctrl-X, Ctrl-V for DNA

Dan Maloney posted: "Once upon a time, the aspiring nerdling's gift of choice was the Gilbert chemistry set. Its tiny vials of reagents, rack of test tubes, and instruction book promised endless intellectual stimulation and the possibility of stink bombs on demand. Now a new " Respond to this post by replying above this line New post on Hackaday Ctrl-X, Ctrl-V for DNA by Dan Maloney Once upon a time, the aspiring nerdling's gift of choice was the Gilbert chemistry set. Its tiny vials of reagents, rack of test tubes, and instruction book promised endless intellectual stimulation and the possibility of stink bombs on demand. Now a new genetic engineering lab-in-a-box Kickstarter, with all the tools and materials needed to create your own transgenic organisms, may help the young biohacker's dreams come true. The Kickstarter has been wildly successful. The initial goal was $1200AUD was met in a day, and currently stands at almost $6200AUD. Despite that success, color me skeptical on this one. Having done way more than my fair share of gene splicing, there seem to be a few critical gaps in this kit. For example, the list of materials for the full kit includes BL21 competent E. coli as the host strain. Those cells are designed to become porous to extracellular DNA when treated with calcium chloride and provided with a heat shock of 42°C. At a minimum I'd think they'd include a thermometer so you can control the heat shock process. Plenty of other steps also need fairly precise incubations, like the digestion and ligation steps needed to get your gene into the host. And exactly what technique you'd be using to harvest DNA from the animal, plant or fungal cells is unclear; the fact that most of the techniques for doing so require special techniques leads me to believe there's a lot less here than meets the eye. To be fair, I've been off the lab bench for the better part of two decades, and the state of the art has no doubt advanced in that time. There could very well be techniques I'm not familiar with that make the various steps needed to transform a bacterial culture with foreign DNA trivial. It could also be the case that the techniques I used in the lab were optimized for yield and for precise data, while the GlowGene kit provides the materials to get a "good enough" result. I hope so, because a kit like this could really expand the horizons of hackerdom and start getting the biohacking movement going. [Thanks, Michael!] Dan Maloney | September 20, 2015 at 7:01 am | Tags: bio hack, biohacking, E coli, gene splicing, genetic, genetic engineering, kickstarter, transgenic | Categories: chemistry hacks, misc hacks | URL: http://wp.me/pk3lN-Iff Comment    See all comments Unsubscribe to no longer receive posts from Hackaday. Change your email settings at Manage Subscriptions. Trouble clicking? Copy and paste this URL into your browser: http://hackaday.com/2015/09/20/ctrl-x-ctrl-v-for-dna/ Thanks for flying with WordPress.com