Re: [DIYbio] Storing Photobacterium Phosporeum

I've lost every stock of Vibrio phosphoreum* I ever made at -20, as has
an academic colleague who made stocks at -20. I'm actually concerned it
might be the opposite of what you say Andreas; because V.phosphoreum is
very cold-tolerant, I have a mild suspicion that it continues a very
basal metabolism when antifrozen at -20, enough to kill itself? I can't
imagine why else it appears so brittle.

I may just be unlucky. BCCing in another DIYbioer who I know to have
isolated these guys who might have had better luck..

* Originally termed "Photobacterium" but since renamed

On 21/09/15 08:25, Mega [Andreas Stuermer] wrote:
> Agar plates in the fridge should still be viable after weeks. At
> least with E coli and agrobacterium even months in my experience. Or
> try making stab culture (in eppis). You can freeze them in 10%
> glycerol or just LB at -15 for less than a year probably at -15°C. I
> think people underestimate the hardiness of bacteria. On the other
> hand, a marine bacterium may not have evolved antifreeze mechanisms
> like a soil/land bacterium
>

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