How about the raw ligase? I buy ATP directly from NEB to do goldengates with, and some ligase from a non-commercial source might be interesting.
-- Also, how are you getting the ligase? Expression of it in house? Or resale? Either or, I'm sure it'll be a great offer, but I am curious if you had the vector in house for some in vivo ligations I'd like to test out. Anyway, useful site! It's awesome that you've gotten more enzymes up, but I think BsaI/BsmBI are also a real important couple. Once you get materials to do GoldenGate, it's a pretty wonderful system, just wish I could get around to making some basic parts for DIY vectors...
-Koeng
On Monday, October 12, 2015 at 1:37:02 PM UTC-7, Josiah Zayner wrote:
On Monday, October 12, 2015 at 1:37:02 PM UTC-7, Josiah Zayner wrote:
My company, The ODIN(http://www.the-odin.com) is starting to ship restriction enzymes and ligase! However, enzymes are pretty heat stable. T4 DNA ligase is another matter, it requires ATP, ATP hydrolyzes. I need a few people to test out some T4 DNA ligase and reaction buffer that has been shipped. Shipping of Taq and other Master Mixes has worked and they contain dNTPs so I assume this should work out just fine but I need testers to make sure.
If you goto the website(http://www.the-odin.com/t4-dna-ligase-5ul/ ) and use coupon code FREETHELIGASE I will ship you some ligase for free as long as you promise to provide feedback on how it works after shipping.
Thanks,
Josiah Zayner
The ODIN
ca...@the-odin.com
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