Re: [DIYbio] automated liquid delivery to a petri dish

For microfluidics, check out the protocol I developed on the SVA-NYC iGEM 2015 wiki page.

http://2015.igem.org/Template:SVA-NYC/Microfluidics

Its still under construction but the protocol is solid and uses simple reagents and off the shelf (except for corona wand) components. MIT's nanomaker lecture series has a cool DIY plasma cleaning chamber but requires a two stage vacuum to maintain the plasma.

https://m.youtube.com/watch?v=fMUemBZ0k5Q

Contact me if you need help! Best of luck!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC


> On Oct 1, 2015, at 3:15 PM, Carlos Castellanos <carloscastellanossf@gmail.com> wrote:
>
> Hey DIYBio-ers,
>
> I am hoping you can offer advice for a project I am working on.
>
> I need to deliver liquid nutrients to a culture of c. elegans nematodes via an Arduino-controlled syringe pump. This syringe pump part is actually done. What I need is some stable hardware to connect the tubing from the syringe (via a luer-locked dispensing needle or similar connector) to different areas of a petri dish. Perhaps something like a perfusion chamber systems like this one:
> http://www.biosciencetools.com/catalog/Fitting_Kit.htm
>
> I've also been looking at building a simple microfluidic chamber.
>
> I also need to be able to monitor all this via a digital microscope, so it must be unobstructed for the top.
>
> I have a rough set-up that I created for testing by simply drilling a small hole into the petri dish to put the dispensing needle through. But I said I need something more stable.
>
> Any ideas or suggestions as to how to proceed?
>
> Thanks
> Carlos
>
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