Re: [DIYbio] Re: Aquiring E.coli lab strains...

You might try Carolina, they cater to educators, and I've had good
luck with them as an individual:
http://www.carolina.com/help/international-ordering

On Wed, Oct 28, 2015 at 10:42 AM, Dr. N00b <drn00b@drn00b.com> wrote:
> E-mail sent. And thanks! :)
>
> On Wednesday, October 28, 2015 at 6:26:11 PM UTC+1, Josiah Zayner wrote:
>>
>> We do ship to Norway but on a hard to find place on the website it
>> indicates that for international shipping to contact us. This is because of
>> customs, shipping costs and other associated issues.
>>
>> Strains I usually ship on filter paper in an envelope which decreases the
>> viability of the strains but removes some shipping cost. Contact me off list
>> and we will get you all setup.
>>
>> Thanks,
>> Josiah Zayner
>> case@the-odin.com
>>
>>
>>
>> On Wed, Oct 28, 2015 at 10:12 AM, Dr. N00b <...> wrote:
>>>
>>> This is absoultely awesome!
>>>
>>> However - I just now tried to place an order for Bioluminescent E.Coli,
>>> E.Coli DH5a and PDusk and PDawn - Light Controlled Fluorescent Protein
>>> Expression - and I got this unpleasant message:
>>>
>>> "Unfortunately one or more items in your cart can't be shipped to your
>>> location. Please choose a different delivery address."
>>>
>>> I just so happen to be located in Norway btw.
>>>
>>> Sincerely, N00b.
>>>
>>> On Wednesday, October 28, 2015 at 5:59:55 PM UTC+1, Josiah Zayner wrote:
>>>>
>>>> Check out http://the-odin.com we cater to the Biohackers!
>>>>
>>>> Josiah Zayner, Ph.D.
>>>>
>>>> On Wednesday, October 28, 2015 at 9:23:54 AM UTC-7, Dr. N00b wrote:
>>>>>
>>>>> Hello everyone!
>>>>>
>>>>> I am a aspiring hobby geneticist - and this will be my first post here.
>>>>> :)
>>>>>
>>>>> I am looking to conduct my first experiment - bacterial transformation,
>>>>> and I am on the lookout for some ampicillin, tetracyclin and/or kanamycin
>>>>> resistant e.coli strains that I can transform using some GFP vectors I have
>>>>> incoming.
>>>>> I will be doing this as a small project in connection with a short
>>>>> article I will be writing for my workplace internal magazine on the topic of
>>>>> genetics and its importance now and in the future.
>>>>>
>>>>> Anyhow, I am finding it very difficult to get any lab strains e.coli at
>>>>> all. All of the big companies that provide such things do not cater to non
>>>>> govt. or educational institutions, and if I call a biobank or lab to ask if
>>>>> they have any strains or plasmids they'd be willing to share - I am simply
>>>>> met with the response "who ARE you? ..." followed by "sorry, we can't sell
>>>>> you any"... After which I think to myself "cant... or wont?"...
>>>>>
>>>>> Well, that's just it... I'm a simple private individual who wants to
>>>>> learn more about genetics. I've bought some literature and got all the
>>>>> neccesary protocols for making everything from Lb broth, agar medium to
>>>>> mini-prep kits for bacterial transformation.
>>>>>
>>>>> Right now a friendly soul has shipped me some strains all the way from
>>>>> the Netherlands, and I have a GFP vector sample on the way to me next week,
>>>>> but in case those don't make it for whatever reason - I am on the lookout
>>>>> for a more reliable source of strains and plasmid vectors for the future - a
>>>>> source that does not discriminate against us private DIY's.... :)
>>>>>
>>>>> Anyhow, a friend of mine urged me to also blog about what I am doing so
>>>>> that perhaps someone else might get inspired to do the same... So if anyone
>>>>> would like to have a look, you can see my progress from the very beginning
>>>>> to right now at www.drn00b.com
>>>>> I've spent the past couple of years buying equipment, and just recently
>>>>> I've managed to get my lab up and running. Only thing missing right now are
>>>>> e.coli strains and vectors.
>>>>>
>>>>> I appreciate any and all input on the matter. :)
>>>>>
>>>>> Sincerely, Dr. N00b.
>>>>>
>>
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--
-Nathan

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