A single pOSIP plasmid (https://www.addgene.org/45985/) will most likely save you 65$ worth of time, and plus you won't have to worry about size. What exactly do you want to integrate?
-- If you still want to insert your gene using lambda red, put it in/on a defective prophage. Qin/Kim, e14, rac, CP4-57, CP4-6, DLP12 prophages would probably all work. (just looked quickly at E coli genome for these)
-Koeng
On Thursday, October 29, 2015 at 4:00:15 AM UTC-7, Linden wrote:
On Thursday, October 29, 2015 at 4:00:15 AM UTC-7, Linden wrote:
Hey Guys!I would like to know how do I pick where in the E. coli chromossome I should integrate my DNA so it doesn´t have any impact on other genes.Thank you for you time!
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