[DIYbio] Re: Power tool analogue in MolBio.

"Biotinylation of a protein is easy peasy" In Vivo?

Just as in any of these I am hesitant about is if the fit is too good and the turnover too slow. From skim readings I understood that there might be some off target activity in both bacterial and mammalian systems by the Biotinylation enzyme.

Looks like I missed a couple of deleted messages, shame. Maybe we've got like minded ideas :)

After doing some further digging I found something called nano-bodies, turns out camels and lamas produce them. I figure with alpaca farms popping up this might be the way to go. They are 15 kDa still bulky but not as bulky as the set of tools I'd like to test them against.

FLAG-tag (1.5 kDa) + M2 (150 kDa?): I just read it. I like this one. I might just try it out. Thank you for bringing that to my attention, Koeng.

Also there seems to be at least two aa antibody target guessing software, given an input. They are called Bishop (download) and AbDesigner (online via NIH website). I am not completely sure how the second one works but I know that the post translational modification and 3D structures are not taken into consideration. Although mods are predicted in output page.

As for the rest, I am still digging through literature on all of that.

The purpose is to make your lives easier. Other than that don't mind the purpose for now. I'll let you know later.

-Yuriy

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