I stand corrected... Happened to come across that paper
"An Optimized microRNA Backbone for Effective Single-Copy RNAi" (open access, too big to attach here http://www.cell.com/cell-reports/fulltext/S2211-1247%2813%2900687-6 )
-- "An Optimized microRNA Backbone for Effective Single-Copy RNAi" (open access, too big to attach here http://www.cell.com/cell-
That's the way how to do it. You need a sense and anti-sense. I didn't see that when BLASTing last time. Snapgene showed me that the sequence is present twice, and I thought that must be an error.
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