Re: [DIYbio] Synthetic biology in Rhodobacter: what a great fun

Sorry for the late reply, got busy in the lab.

@Kate Thanks for the interest, as Brian suggests let's keep this conversation public, that was my first aim formerly. If you feel like the topic you want to discuss are sensible or you wanna keep them by yourself drop me a line (you can send messages privately).

Regarding the transformation I agree, it's a tough cookie. But what a delicious filling.

It may be "difficult" to introduce foreign material in it but you have chance to directly integrate the dna into the genome or to keep it episomal.

In this article http://goo.gl/iZPFWP transformation procedures are adressed. Well, just conjugation.

In this case I would always suggest to perform genomic integration, as it is much stable and less prone to diffusion.

@Dennis It definitely looks interesting from the setup point of view but it looks even more promising if somebody would like to use it to setup real production of molecules or for bioprocess development. These bacteria are commonly used in wastewater treatment, nonetheless as they are GRAF they are used as supplement in animal feed.

Apparently, but I should look better into that, they have some kind of stimulant effect also on plants.

Applications are p[lenty, just by using a wild type organism. But I am more interested in what you could actually produce by using it as a chassis for synthetic biology. Potentially they could grow on lignocellulosic biomass derived sugars, or express extracellular proteins to degrade cellulosic compounds.

They can live off of methanol, that you could synthesize cheaply and also integrate their diet with CO2 ans light.

Again would be awesome to find somebody with lab experience on this subject and delve into the details :)

Or come up with a project on this group and develop it further, why not?

Best,

Fede

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