Re: [DIYbio] Synthetic biology in Rhodobacter: what a great fun

Here is a paper from 2014 which claims that chemical or electroporative transformation does not work in Rhodobacter. They only where able to do it using conjugative transformation.

If you decide to follow the conjugation path, at least in USA, using e.coli capable of horizontal gene transfer requires you to follow the NIH Guidelines. Other people in this forum, who are more familiar with the topic, could better explain what it means.

Transformation of Rhodobacter in DIYBio setup is probably an interesting project by itself. Please, share you results if you get somewhere.

On Mon, Nov 16, 2015 at 5:03 AM Brian Degger <brian.degger@gmail.com> wrote:
What are you interested in Kate?
It's somewhat nice to keep the conversation open on this diybio forum. Especially in the start. 
I can understand that you might have something to offer on the subject. And we(I use the royal we, I speak for myself and a few friends on DIYBio that I know well) would all love to hear it. We are interested in everything, and debating and finding out more. We are semi-dogmatic when it comes to self-gm in a kitchen(against), and (against) anything about using biology for evil, or letting people do damage by bio-error-ism after they have contacted the group. 

Also.. you give your personal email out to 4406+ people. I would be fine with that, because other people are always publishing my email to others and I want people to look at my public Google+ profile. You might not be so happy about that. 

Cheers,
Brian



On Mon, Nov 16, 2015 at 5:07 AM, Katherine Gordon <kthrngordon@gmail.com> wrote:

Please contact me directly at kthrngordon@gmail.com my name is Kate.

On Nov 15, 2015 10:04 PM, "Katherine Gordon" <kthrngordon@gmail.com> wrote:

Rewiring metabolism. Brilliant!
On Nov 15, 2015 1:30 PM, "BraveScience" <bravescience@gmail.com> wrote:
>
> Hi,
>
> Recently I started being interested in purple bacteria. More specifically I found Rhodobacter spheroides a very interesting organism.
> It has all the gigs as a metabolic engineer you'd like to have from your chassis: heterotrophism, chemoautotrophism and photoheterotrophism.
> I work with cyanobacteria for living but these purple beauties got my full attention.
>
> It lives in waste water, loves all sugars (xylose from biomass isn't gonna be a problem, if it is we can fix it easily) and can live off the most incredible substrates, from methanol, H2 to poop.
>
> Most importantly it's very pretty with all those red colors.
>
> As I am looking into potential synthetic biological application, and metabolism rewiring and modelling, I was wondering if anybody has work before with such beautiful organism.
>
>
> Find it interesting or have any heads up where to look? Please, drop me a line.
>
> Best,
> Fede

Hello Fede,
I studied biochemistry in. College in the late 90,s. Mitochondria were and still are my interest . You mention rewiring metabolism...How would you go about this trick? I would greatly be interested in your thoughts. A symbiotic relationship between these bacterial meto bolic engineers and our somatic cells is possibly the most remarkable thing Ive ever heard.
>
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twitter: @drbrian

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