[DIYbio] Re: DIY Electroporator (invitrogen II) - reverse engineered manual/documentation

This is excellent work.  I would have expected " Modification 2 " to be part of the original circuit in a hv supply, surprised that it's not, seems like a good modification.  What's your thoughts on wearing a ground strap on one wrist during use?   HV experiments, definitely one-handed.  But undecided on whether a ground strap would be beneficial.  HV hookup wire might be better for the leads from the power supply.  Perhaps the manual knobs can be eliminated and use switch IC's instead.  It would be good to add photos of the cuvettes for completeness.  You might consider publishing this in BioCoder if you haven't already published somewhere.  As for the protocols it seems like the choice of buffer is almost more of an important consideration for higher yield than the voltage choice or waveform shape, from reading published comparison results.



## Jonathan Cline
## jcline@ieee.org
## Mobile: +1-805-617-0223
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On Thursday, September 10, 2015 at 10:58:49 AM UTC-7, cameron wrote:

Folks,

I bought an old Invitrogen Electroporator on Ebay for $65. There wasn't a manual so one of our members reverse engineered it and created a wiki detailing how it works. Graham Widerman also found some flaws in the design so he replaced some of the components. For those who want to build your own electroportator, this is a great document to use. We also built in leads which allows us to hook up an oscilloscope directly to monitor the wave form during electroporations. Find the wiki HERE. This is the only place on the web where information can be found on this electroporator so please share as needed.

Cameron

http://grahamwideman.wikispaces.com/Invitrogen+Electroporator+II