Hi,
This is a very cool project, kudos to you guys.
I have been developing a similar pipeline myself, but I started with the growing part and building automated bioreactors first to grow the algae in before I start with any transfections. If you think I can help you in any way just let me know, it'd be great to team up with like-minded people.
My first thought when looking at your project was the same as Fede's: I would imagine using cyanobacteria would make your life a lot easier. That's what I am planning anyway...
My second was that insulin will just get digested if eaten with algae, and purifying it from chloroplasts would probably be more expensive than the current production methods. Also, oral vaccines tend to be entire virus capsids or even bacteria spores not single proteins that are expressed alone - although I admit I don't know the topic really well, so I am happy for any education on this. My idea was to express GFP first as a proof-of-concept, and then try to build some flavour compounds (although I haven't actually chosen any as they too seem rather difficult).
Either way, if you are interested in further discussion, I'd be very to talk to you guys about more details.
Cheers,
Mate
On Sunday, 6 December 2015 23:31:33 UTC, antomicblitz wrote:
This is a very cool project, kudos to you guys.
I have been developing a similar pipeline myself, but I started with the growing part and building automated bioreactors first to grow the algae in before I start with any transfections. If you think I can help you in any way just let me know, it'd be great to team up with like-minded people.
My first thought when looking at your project was the same as Fede's: I would imagine using cyanobacteria would make your life a lot easier. That's what I am planning anyway...
My second was that insulin will just get digested if eaten with algae, and purifying it from chloroplasts would probably be more expensive than the current production methods. Also, oral vaccines tend to be entire virus capsids or even bacteria spores not single proteins that are expressed alone - although I admit I don't know the topic really well, so I am happy for any education on this. My idea was to express GFP first as a proof-of-concept, and then try to build some flavour compounds (although I haven't actually chosen any as they too seem rather difficult).
Either way, if you are interested in further discussion, I'd be very to talk to you guys about more details.
Cheers,
Mate
On Sunday, 6 December 2015 23:31:33 UTC, antomicblitz wrote:
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