Just ran some rubisco large subunit primers on a bunch of plants I isolated earlier today. I got a 5mL master mix from Gene and Cell Technologies on eBay. Works a treat. Never an issue. Its just taq not pfu but I've even done larger amplicon pcr and sequence came back spotless. The empty or missing bands were from samples with too little or no DNA present. Qubit 2.0 confirmed the claim.
Sebastian S. Cocioba
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Blog: ATinyGreenCell.com
I'd rather pay an extra $20 and get Taq from NEB vs some rando off ebay. But show me the gels and I might be a believer.--On Tue, Dec 22, 2015 at 12:24 AM, Koeng <koeng101@gmail.com> wrote:http://www.ebay.com/itm/Taq-DNA-Polymerase-UltraPure-Grade-HPLC-purified-1-000-units-/301250297676?hash=item4623eabf4c:g:ZOkAAOSwQItTzDq7--and what I found for taq. I haven't used either one of these options.-Koeng
On Monday, December 21, 2015 at 9:23:13 PM UTC-8, Koeng wrote:http://www.aasinc.co/store-and-promotions.html#!/EasyPfu-DNA-Polymerase/p/52930720/category=13912399this looks pretty good
On Monday, December 21, 2015 at 3:26:42 PM UTC-8, Koeng wrote:HeyI have been shopping for some inexpensive polymerase (high fidelity (pfu and such) and taq), and wanted to know if anyone here knows of an ultra-cheap supplier. Thanks-Koeng
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