The algae is washed and resuspended in 375 mM sorbitol. The biorad gene pulser ii electroporation settings we use for a 2 mm cuvette are 2.2 kv, 600 ohms, and 50 uf, and I think the pulse is an exponential decay. We use a single pulse and get good transformations with time constants from 20-30 ms.
On Saturday, December 19, 2015 at 12:56:55 PM UTC-5, John Griessen wrote:
-- On Saturday, December 19, 2015 at 12:56:55 PM UTC-5, John Griessen wrote:
On 12/19/2015 10:51 AM, poli wrote:
> The nanno oceanica I work with
Is your suspension for electroporation salty and conductive? What kind of voltage and waveshape and number of repetitions
do you use?
I'm interested for purposes of designing low-cost-super-safe electroporation equipment. So far, the common uses
are for non-salty suspensions so that very low power pulses work. If salty and conductive, most of the energy goes into
warming up the solution and pulls volts down unless you supply much more energy to the suspension.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/348f9bc9-ea14-4d4f-aa88-431ab0e804b0%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment