Re: [DIYbio] Re: Electroporation vs Sonification vs CaCl Chlorella Vulgrais

You said you're going to use CMV promoter? Better don't, it seems to get confused with CaMV promotor (35S) from time to time. The former one is from the latent Cytomegalovirus which infects mammals and works in vertebrates and insects, and the other one from the Cauliflower mosaic virus which is active in plants.

Wouldn't it be easier to start with pCambia1302? It already contains all the elements you need.

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