I am not pursuing of ''zapping myself'' so i think those calculations are irrelevant, besides that I never seen anyone touching cuvette with two hands and if it will be touched with one the current will go straight from one finger to another and back to the cathode. And while I have access to brand new $8000 piece of equipment I dont really see a point of getting some molded rusty box of a cat in a sack. Besides i can build sonicator myself for under $50 so i am deffinately not spending hundreds on electroporator.
On Friday, December 18, 2015 at 4:18:55 PM UTC-8, John Griessen wrote:
-- On Friday, December 18, 2015 at 4:18:55 PM UTC-8, John Griessen wrote:
On 12/18/2015 08:50 AM, Alex D wrote:
> Theoretically if buffer is present and just by placing a 50cent cuvette into the electrophoretic chamber it should work.
Have you analyzed that with the resistivity concept? Where longer paths made of tiles of the same resistivity square
of material have higher and higher resistance? Sounds like a possible way to get zapped while fiddling with unknowns and
getting in a hurried mood.
We do
> have functional electroporator from BTX but i was trying to figure out the way to avoid using it due to its price.
Is its price in the range of the super cheap < $200 ebay prices for Biorad "classical" electroporators?
If so, that's cheap enough.
Going lower is OT for this list, isn't it?
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