Yea i have noticed that actually, and yea we can use many common plasmids but we probably will just order custom to avoid restriction use as much as possible. Recent study showed that chlorellas chromosomes can replicated in mammalian cells too and use of the promoter doesnt induce any infections or posses threat. SV40 promoter was used for many years in gm fish many years back
On Saturday, December 19, 2015 at 4:16:39 AM UTC-8, Mega [Andreas Stuermer] wrote:
-- On Saturday, December 19, 2015 at 4:16:39 AM UTC-8, Mega [Andreas Stuermer] wrote:
You said you're going to use CMV promoter? Better don't, it seems to get confused with CaMV promotor (35S) from time to time. The former one is from the latent Cytomegalovirus which infects mammals and works in vertebrates and insects, and the other one from the Cauliflower mosaic virus which is active in plants.Wouldn't it be easier to start with pCambia1302? It already contains all the elements you need.
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