Re: [DIYbio] Re: Electroporation vs Sonification vs CaCl Chlorella Vulgrais

On 12/19/2015 10:51 AM, poli wrote:
> The nanno oceanica I work with

Is your suspension for electroporation salty and conductive? What kind of voltage and waveshape and number of repetitions
do you use?

I'm interested for purposes of designing low-cost-super-safe electroporation equipment. So far, the common uses
are for non-salty suspensions so that very low power pulses work. If salty and conductive, most of the energy goes into
warming up the solution and pulls volts down unless you supply much more energy to the suspension.

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