Re: [DIYbio] How do I make Chamomile produce the chemical Harmala alkaloid?

Find the genes you need, pcr synthesize and assemble the genes into pCambia vector (2301 is nice since it has GUS and the more nooby friendly kanamycin resistance gene), obtain an agrobacterium tumefaciens strain from local University, heat shock final plasmid in, develop somatic embryogenesis protocol for cammomile or google for agrobacterium mediated transformation of cammomile research paper, duplicate methods, pray, pcr confirm, party.

Sebastian S. Cocioba

CEO & Founder

New York Botanics, LLC

Blog: ATinyGreenCell.com

On Jan 27, 2016, at 1:10 PM, 'Erik Robb' via DIYbio <diy...@googlegroups.com> wrote:

I need a step by step process to make this happen. In an effort to make a more relaxing tea I have secured funding to make this project work. The only problem is that I need a step by step process , even if its not detailed at all, I can figure it out. It's really hard to find help with this so if anyone has the knowledge to give me a step by step I really need it. Assume I have all the skills necessary because i do, just need a step by step.

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