It depends on the radioactive isotope used. Some isotopes emit alpha or beta particles, but some emit gamma photons. The most cost effective way to expose cells to ionizing EM radiation would be to leave it outside in the sun. You get a relatively small dose of ionizing UV from sunlight, might not be enough for these experiments, but direct sunlight tends to be fairly damaging to DNA (hence melanoma). Would the damage from gamma rays be the same as UV? They are both ionizing EM radiation, but gamma rays have a lot more energy.
Another, more controllable source of UV would be tanning bed lights.
Another, more controllable source of UV would be tanning bed lights.
On Fri, Jan 29, 2016 at 2:38 PM Dennis Oleksyuk <mail@dennis-o.com> wrote:
Now this become curious. How would you test radiation resistance? What would be the most cost efficient way to expose cell culture to ionizing radiation?I bet that radioactive isotopes is off the table for majority of places. Can x-ray radiation used? Is it different in its DNA damage properties from radiation from radioactive isotopes? If so, then some old x-ray machine probably good the job.Is there any alternative?--On Fri, Jan 29, 2016 at 3:15 PM Koeng <koeng101@gmail.com> wrote:"This proteogenomic study also indicates that the high radiotolerance of T. gammatolerans is probably due to proteins that remain to be characterized"Direct injection of proteins will likely do more harm than good, and they don't even know the exact proteins that confer to their resistance. Finding the *exact* genes is the first task
On Thursday, January 28, 2016 at 3:59:23 PM UTC-8, Finn Daffron wrote:--Thank you for your help and the link. I have, based on information provided in the paper and by you, determined a method that does not involve live thermococcus gammatolerans but instead the recombinant proteins that provide DNA repair in thermococcus gammatolerans.
On Thursday, January 28, 2016 at 3:11:22 PM UTC-5, Finn Daffron wrote:I am trying to create a batch of thermococcus gammatolerans. This is because I want to use CRISPR to inject thermococcus gammatolerans into rats in order to create radiation resistance with possible implications being people working in nuclear power plants, astronauts, etc. Does anyone have any ideas on how it would be synthesized? Please get back to me.
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